recombinant proteins rs 504393 tocris Search Results


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Tocris recombinant proteins rs 504393 tocris
Recombinant Proteins Rs 504393 Tocris, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cosmo Bio USA mouse recombinant mcp-5
Mouse Recombinant Mcp 5, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biorbyt rabbit polyclonal anti ccr2
(A) Scheme of RS-504393 treatment at different DMM-induced OA stages. (B) Protein levels of Collagen 10 (Col10), Osteocalcin (OCN) and MMP-13 were measured by IHC in medial femurs and tibiae obtained from mice 4 weeks after DMM/sham, with or without treatment with the <t>CCR2</t> antagonist, RS-504393, from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described (C) mRNA expression of Col10 and OCN was evaluated by in-situ hybridization (ISH) on DMM/sham knees, with or without RS-504393 treatment from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described. (D) OARSI score was obtained from sections (ranging between 8 and 12 per mouse) from DMM-operated knees at 4, 8 and 12 weeks after surgery with or without RS-504393 treatment at the time indicated (N=6 for each of the experimental points described). The graph represents the mean ± standard deviation. Significance was set at P < 0.05. (E) Representative sections of OARSI score are shown at the indicated time points (N=6 for each of the experimental points described). Scale bars are 100 µm.
Rabbit Polyclonal Anti Ccr2, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10X Genomics reagent kits v2 10x genomics
(A) Scheme of RS-504393 treatment at different DMM-induced OA stages. (B) Protein levels of Collagen 10 (Col10), Osteocalcin (OCN) and MMP-13 were measured by IHC in medial femurs and tibiae obtained from mice 4 weeks after DMM/sham, with or without treatment with the <t>CCR2</t> antagonist, RS-504393, from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described (C) mRNA expression of Col10 and OCN was evaluated by in-situ hybridization (ISH) on DMM/sham knees, with or without RS-504393 treatment from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described. (D) OARSI score was obtained from sections (ranging between 8 and 12 per mouse) from DMM-operated knees at 4, 8 and 12 weeks after surgery with or without RS-504393 treatment at the time indicated (N=6 for each of the experimental points described). The graph represents the mean ± standard deviation. Significance was set at P < 0.05. (E) Representative sections of OARSI score are shown at the indicated time points (N=6 for each of the experimental points described). Scale bars are 100 µm.
Reagent Kits V2 10x Genomics, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bethyl resource source identifier antibodies goat albumin bethyl
(A) Scheme of RS-504393 treatment at different DMM-induced OA stages. (B) Protein levels of Collagen 10 (Col10), Osteocalcin (OCN) and MMP-13 were measured by IHC in medial femurs and tibiae obtained from mice 4 weeks after DMM/sham, with or without treatment with the <t>CCR2</t> antagonist, RS-504393, from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described (C) mRNA expression of Col10 and OCN was evaluated by in-situ hybridization (ISH) on DMM/sham knees, with or without RS-504393 treatment from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described. (D) OARSI score was obtained from sections (ranging between 8 and 12 per mouse) from DMM-operated knees at 4, 8 and 12 weeks after surgery with or without RS-504393 treatment at the time indicated (N=6 for each of the experimental points described). The graph represents the mean ± standard deviation. Significance was set at P < 0.05. (E) Representative sections of OARSI score are shown at the indicated time points (N=6 for each of the experimental points described). Scale bars are 100 µm.
Resource Source Identifier Antibodies Goat Albumin Bethyl, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Scheme of RS-504393 treatment at different DMM-induced OA stages. (B) Protein levels of Collagen 10 (Col10), Osteocalcin (OCN) and MMP-13 were measured by IHC in medial femurs and tibiae obtained from mice 4 weeks after DMM/sham, with or without treatment with the CCR2 antagonist, RS-504393, from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described (C) mRNA expression of Col10 and OCN was evaluated by in-situ hybridization (ISH) on DMM/sham knees, with or without RS-504393 treatment from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described. (D) OARSI score was obtained from sections (ranging between 8 and 12 per mouse) from DMM-operated knees at 4, 8 and 12 weeks after surgery with or without RS-504393 treatment at the time indicated (N=6 for each of the experimental points described). The graph represents the mean ± standard deviation. Significance was set at P < 0.05. (E) Representative sections of OARSI score are shown at the indicated time points (N=6 for each of the experimental points described). Scale bars are 100 µm.

Journal: Osteoarthritis and cartilage

Article Title: Role of the C-C chemokine Receptor-2 in a Murine Model of Injury-induced Osteoarthritis

doi: 10.1016/j.joca.2016.11.004

Figure Lengend Snippet: (A) Scheme of RS-504393 treatment at different DMM-induced OA stages. (B) Protein levels of Collagen 10 (Col10), Osteocalcin (OCN) and MMP-13 were measured by IHC in medial femurs and tibiae obtained from mice 4 weeks after DMM/sham, with or without treatment with the CCR2 antagonist, RS-504393, from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described (C) mRNA expression of Col10 and OCN was evaluated by in-situ hybridization (ISH) on DMM/sham knees, with or without RS-504393 treatment from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described. (D) OARSI score was obtained from sections (ranging between 8 and 12 per mouse) from DMM-operated knees at 4, 8 and 12 weeks after surgery with or without RS-504393 treatment at the time indicated (N=6 for each of the experimental points described). The graph represents the mean ± standard deviation. Significance was set at P < 0.05. (E) Representative sections of OARSI score are shown at the indicated time points (N=6 for each of the experimental points described). Scale bars are 100 µm.

Article Snippet: Rabbit polyclonal anti-CCL2 (orb13563), CCL8 (orb13565), CCL7 (orb13567), CCL13 (orb13285) and CCL12 (orb13284) antibodies were from Biorbyt; rabbit polyclonal anti-CCR2 was from Novusbio (NB100-701); anti-CollagenX (ab58632), anti-Osteocalcin (ab10911) and mouse monoclonal anti-MMP-13 (ab3208) antibodies were from Abcam; The CCR-2 antagonist RS-504393 was from Tocris Bioscience.

Techniques: Expressing, In Situ Hybridization, Standard Deviation

Levels of (A) CCL12 and (B) other’s CCR2 ligands (CCL2, CCL8, CCL7, CCL13) were measured by immunohistochemistry (IHC) in articular cartilage sections obtained from medial knee joints of mice at the indicated time points after DMM or sham control surgery. Brown is immunopositive staining. DMM-operated knees at 4, 8 and 12 weeks post-surgery were immunostained with antibodies to CCR2 and protein levels where visualized in (C) medial tibial cartilage, (D) chondrocytes of the growth plate and (E) synovium. Images are representative of 6 different mice for each of the experimental points described. Scale bars are 100 µm.

Journal: Osteoarthritis and cartilage

Article Title: Role of the C-C chemokine Receptor-2 in a Murine Model of Injury-induced Osteoarthritis

doi: 10.1016/j.joca.2016.11.004

Figure Lengend Snippet: Levels of (A) CCL12 and (B) other’s CCR2 ligands (CCL2, CCL8, CCL7, CCL13) were measured by immunohistochemistry (IHC) in articular cartilage sections obtained from medial knee joints of mice at the indicated time points after DMM or sham control surgery. Brown is immunopositive staining. DMM-operated knees at 4, 8 and 12 weeks post-surgery were immunostained with antibodies to CCR2 and protein levels where visualized in (C) medial tibial cartilage, (D) chondrocytes of the growth plate and (E) synovium. Images are representative of 6 different mice for each of the experimental points described. Scale bars are 100 µm.

Article Snippet: Rabbit polyclonal anti-CCL2 (orb13563), CCL8 (orb13565), CCL7 (orb13567), CCL13 (orb13285) and CCL12 (orb13284) antibodies were from Biorbyt; rabbit polyclonal anti-CCR2 was from Novusbio (NB100-701); anti-CollagenX (ab58632), anti-Osteocalcin (ab10911) and mouse monoclonal anti-MMP-13 (ab3208) antibodies were from Abcam; The CCR-2 antagonist RS-504393 was from Tocris Bioscience.

Techniques: Immunohistochemistry, Staining

Protein levels of CCL12 were visualized by IHC in the bone tissues (A) 2 wks after DMM/sham, as well as (B) 4 and 8 wks post-surgery. Protein levels of (C) CCL2, CCL8, CCL7, CCL13, as well as (D) CCR2 were visualized in bone tissue by IHC at the indicated time points. (E) Protein levels and mRNA expression of Col10 were visualized by IHC and ISH, respectively, in medial femurs obtained from mice 1wk after DMM/sham surgery. Osteophytes were visualized by (F) μCT analyses and (G) OCN protein/mRNA levels (IHC and ISH) in medial femurs and tibiae obtained from mice 2wks after DMM/sham surgery. Images are representative of 6 different mice for each of the experimental points described. Scale bars are 100 µm.

Journal: Osteoarthritis and cartilage

Article Title: Role of the C-C chemokine Receptor-2 in a Murine Model of Injury-induced Osteoarthritis

doi: 10.1016/j.joca.2016.11.004

Figure Lengend Snippet: Protein levels of CCL12 were visualized by IHC in the bone tissues (A) 2 wks after DMM/sham, as well as (B) 4 and 8 wks post-surgery. Protein levels of (C) CCL2, CCL8, CCL7, CCL13, as well as (D) CCR2 were visualized in bone tissue by IHC at the indicated time points. (E) Protein levels and mRNA expression of Col10 were visualized by IHC and ISH, respectively, in medial femurs obtained from mice 1wk after DMM/sham surgery. Osteophytes were visualized by (F) μCT analyses and (G) OCN protein/mRNA levels (IHC and ISH) in medial femurs and tibiae obtained from mice 2wks after DMM/sham surgery. Images are representative of 6 different mice for each of the experimental points described. Scale bars are 100 µm.

Article Snippet: Rabbit polyclonal anti-CCL2 (orb13563), CCL8 (orb13565), CCL7 (orb13567), CCL13 (orb13285) and CCL12 (orb13284) antibodies were from Biorbyt; rabbit polyclonal anti-CCR2 was from Novusbio (NB100-701); anti-CollagenX (ab58632), anti-Osteocalcin (ab10911) and mouse monoclonal anti-MMP-13 (ab3208) antibodies were from Abcam; The CCR-2 antagonist RS-504393 was from Tocris Bioscience.

Techniques: Expressing