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Image Search Results
Journal: Osteoarthritis and cartilage
Article Title: Role of the C-C chemokine Receptor-2 in a Murine Model of Injury-induced Osteoarthritis
doi: 10.1016/j.joca.2016.11.004
Figure Lengend Snippet: (A) Scheme of RS-504393 treatment at different DMM-induced OA stages. (B) Protein levels of Collagen 10 (Col10), Osteocalcin (OCN) and MMP-13 were measured by IHC in medial femurs and tibiae obtained from mice 4 weeks after DMM/sham, with or without treatment with the CCR2 antagonist, RS-504393, from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described (C) mRNA expression of Col10 and OCN was evaluated by in-situ hybridization (ISH) on DMM/sham knees, with or without RS-504393 treatment from 1-to-4wks post-surgery. Images are representative of 6 different mice for each of the experimental points described. (D) OARSI score was obtained from sections (ranging between 8 and 12 per mouse) from DMM-operated knees at 4, 8 and 12 weeks after surgery with or without RS-504393 treatment at the time indicated (N=6 for each of the experimental points described). The graph represents the mean ± standard deviation. Significance was set at P < 0.05. (E) Representative sections of OARSI score are shown at the indicated time points (N=6 for each of the experimental points described). Scale bars are 100 µm.
Article Snippet: Rabbit polyclonal anti-CCL2 (orb13563), CCL8 (orb13565), CCL7 (orb13567), CCL13 (orb13285) and CCL12 (orb13284) antibodies were from
Techniques: Expressing, In Situ Hybridization, Standard Deviation
Journal: Osteoarthritis and cartilage
Article Title: Role of the C-C chemokine Receptor-2 in a Murine Model of Injury-induced Osteoarthritis
doi: 10.1016/j.joca.2016.11.004
Figure Lengend Snippet: Levels of (A) CCL12 and (B) other’s CCR2 ligands (CCL2, CCL8, CCL7, CCL13) were measured by immunohistochemistry (IHC) in articular cartilage sections obtained from medial knee joints of mice at the indicated time points after DMM or sham control surgery. Brown is immunopositive staining. DMM-operated knees at 4, 8 and 12 weeks post-surgery were immunostained with antibodies to CCR2 and protein levels where visualized in (C) medial tibial cartilage, (D) chondrocytes of the growth plate and (E) synovium. Images are representative of 6 different mice for each of the experimental points described. Scale bars are 100 µm.
Article Snippet: Rabbit polyclonal anti-CCL2 (orb13563), CCL8 (orb13565), CCL7 (orb13567), CCL13 (orb13285) and CCL12 (orb13284) antibodies were from
Techniques: Immunohistochemistry, Staining
Journal: Osteoarthritis and cartilage
Article Title: Role of the C-C chemokine Receptor-2 in a Murine Model of Injury-induced Osteoarthritis
doi: 10.1016/j.joca.2016.11.004
Figure Lengend Snippet: Protein levels of CCL12 were visualized by IHC in the bone tissues (A) 2 wks after DMM/sham, as well as (B) 4 and 8 wks post-surgery. Protein levels of (C) CCL2, CCL8, CCL7, CCL13, as well as (D) CCR2 were visualized in bone tissue by IHC at the indicated time points. (E) Protein levels and mRNA expression of Col10 were visualized by IHC and ISH, respectively, in medial femurs obtained from mice 1wk after DMM/sham surgery. Osteophytes were visualized by (F) μCT analyses and (G) OCN protein/mRNA levels (IHC and ISH) in medial femurs and tibiae obtained from mice 2wks after DMM/sham surgery. Images are representative of 6 different mice for each of the experimental points described. Scale bars are 100 µm.
Article Snippet: Rabbit polyclonal anti-CCL2 (orb13563), CCL8 (orb13565), CCL7 (orb13567), CCL13 (orb13285) and CCL12 (orb13284) antibodies were from
Techniques: Expressing